[特邀报告]SETDB1 regulates short interspersed nuclear elements and chromatin loop organization in mouse neural precursor cells

SETDB1 regulates short interspersed nuclear elements and chromatin loop organization in mouse neural precursor cells
编号:103 稿件编号:5 访问权限:仅限参会人 更新:2024-10-27 17:30:47 浏览:296次 特邀报告

报告开始:2024年11月02日 17:30 (Asia/Shanghai)

报告时间:15min

所在会议:[S6] 分会场三:三维基因组成像 / 三维基因组调控 » [s6-1] 分会场三:三维基因组成像 / 三维基因组调控

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摘要
Transposable elements play a critical role in maintaining genome architecture during neurodevelopment. Short Interspersed Nuclear Elements (SINEs), a major subtype of transposable elements, are known to harbor binding sites for the CCCTC-binding factor (CTCF) and pivotal in orchestrating chromatin organization. However, the regulatory mechanisms controlling the activity of SINEs in the developing brain remains elusive. In this study, we conduct a comprehensive genome-wide epigenetic analysis in mouse neural precursor cells using ATAC-seq, ChIP-seq, whole genome bisulfite sequencing, in situ Hi-C, and RNA-seq. Our findings reveal that the SET domain bifurcated histone lysine methyltransferase 1 (SETDB1)-mediated H3K9me3, in conjunction with DNA methylation, confines chromatin accessibility on a selective subset of SINEs in neural precursor cells. Mechanistically, loss of Setdb1 increases CTCF access to these SINE elements and contributes to chromatin loop reorganization. Moreover, de novo loop formation contributes to differential gene expression, including the dysregulation of genes enriched in mitotic pathways. This leads to the disruptions of cell proliferation in the embryonic brain after genetic ablation of Setdb1 both in vitro and in vivo. In summary, our study sheds light on the epigenetic regulation of SINEs in mouse neural precursor cells, suggesting their role in maintaining chromatin organization and cell proliferation during neurodevelopment.
 
关键字
transposable elements, SETDB1, H3K9me3, DNA methylation, chromatin loop, neurodevelopment
报告人
江燕 (Yan Jiang)
复旦大学脑科学研究院 (Institutes of Brain Science, Fudan University)

稿件作者
江燕 复旦大学脑科学研究院
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