[Poster Presentation]SETDB1 regulates short interspersed nuclear elements and chromatin loop organization in mouse neural precursor cells

SETDB1 regulates short interspersed nuclear elements and chromatin loop organization in mouse neural precursor cells
ID:148 Submission ID:24 View Protection:ATTENDEE Updated Time:2024-10-28 14:15:03 Hits:117 Poster Presentation

Start Time:Pending (Asia/Shanghai)

Duration:Pending

Session:[No Session] » [No Session Block]

No files

Abstract
Transposable elements play a critical role in maintaining genome architecture during neurodevelopment. Short Interspersed Nuclear Elements (SINEs), a major subtype of transposable elements, are known to harbor binding sites for the CCCTC-binding factor (CTCF) and pivotal in orchestrating chromatin organization. However, the regulatory mechanisms controlling the activity of SINEs in the developing brain remains elusive. In our study, we conduct a comprehensive genome-wide epigenetic analysis in mouse neural precursor cells using ATAC-seq, ChIP-seq, whole genome bisulfite sequencing, in situ Hi-C, and RNA-seq. Our findings reveal that the SET domain bifurcated histone lysine methyltransferase 1 (SETDB1)-mediated H3K9me3, in conjunction with DNA methylation, restricts chromatin accessibility on a selective subset of SINEs in neural precursor cells. Mechanistically, loss of Setdb1 increases CTCF access to these SINE elements and contributes to chromatin loop reorganization. Moreover, de novo loop formation contributes to differential gene expression, including the dysregulation of genes enriched in mitotic pathways. This leads to the disruptions of cell proliferation in the embryonic brain after genetic ablation of Setdb1 both in vitro and in vivo. In summary, our study sheds light on the epigenetic regulation of SINEs in mouse neural precursor cells, suggesting their role in maintaining chromatin organization and cell proliferation during neurodevelopment.
Keywords
transposable elements,SETDB1,H3K9me3,DNA methylation,chromatin loop,neurodevelopment
Speaker
孙戴静
博士研究生 复旦大学脑科学研究院

Submission Author
孙戴静 复旦大学脑科学研究院
朱月妍 复旦大学脑科学研究院
彭文竹 复旦大学脑科学研究院
郑胜辉 复旦大学脑科学研究院
翁洁 上海市精神卫生中心
董书龙 复旦大学脑科学研究院;复旦大学基础医学院
李嘉琪 复旦大学脑科学研究院
陈颀 复旦大学脑科学研究院
葛传慧 复旦大学脑科学研究院
廖立勇 复旦大学脑科学研究院
董宇昊 复旦大学脑科学研究院
刘赟 复旦大学脑科学研究院;复旦大学基础医学院
蒙伟达 复旦大学脑科学研究院;复旦大学基础医学院
江燕 复旦大学脑科学研究院
Comment submit
Verification code Change another
All comments
Registration Submission